Fig. 6. Suppression of Maxi-Cl currents by a synthetic ANXA2 peptide, Ac-(1-14), but not by the mutated peptide, L7E Ac-(1-14), in C127 cells. A, B. The effects of intracellular application of synthetic ANXA2 and mutated peptides on Maxi-Cl activity. Upper panels show the representative time courses of activation of Maxi-Cl currents after patch excision from C127 cells treated with a mutated synthetic peptide, L7E Ac-(1-14), as negative control (A) and a synthetic ANXA2 peptide, Ac-(1-14) (B), which disrupts the ANXA2-S100A10 complex formation. Ac-(1-14) induced suppression (left: n=6 of 12) or abolition (right: n=6 of 12) of Maxi-Cl activity. During the records, alternating pulses from 0 to ±25 mV were applied. Both peptides were added to bath (intracellular) solution before patch excision. Lower panels show the voltage-dependent inactivation pattern of Maxi-Cl currents elicited by applying voltage pulses (500 ms) from 0 to ±50 mV in 10-mV increments at the time points indicated by triangles in upper panels. C. Summary of the effects of Ac-(1-14) (Ac) and its mutant (L7E-Ac) on Maxi-Cl currents recorded at +25 mV. Each column represents the mean ± SEM (vertical bar). *p<0.05 (Student's t-test) vs L7E-Ac.